ABSTRACT
Objective To observe the changes of MMP-1 and TIMP-1 expression in the pathological scar tissue after scar-skin replantation and to explore the mechanism of treating pathological scars with scar-skin replantation through a rabbit ear model.Methods Rabbit ears were used to establish the hypertrophic scar animal model in this study.Specimens were taken for three times:normal skin,hypertrophic scar and scar-skin replantion separately.We then performed HE staining,Masson staining and immunohistochemical staining to observe the expression of MMP-1 and TIMP-1 in these three groups of specimens.Results The expression of both MMP-1 and TIMP-1 significantly increased in the hypertrophic scar tissue after scar-skin replantation compared with the control group (P＜0.01).The expression of MMP-1 increased more significantly than that of TIMP-1 (P＜0.01).Conclusions The mechanism of scar-skin replantation's effect in the treatment for hypertrophic scar is relevant to the imbalance in the interaction between MMP-1 and TIMP-1 in the scar tissue.
ABSTRACT
Los niveles séricos de la metaloproteinasa (MMP) 9, enzima involucrada en inflamación, y su contraparte, el inhibidor tisular de la metaloproteinasas tipo 1 (TIMP-1), se encuentran significativamente (P < 0,01) incrementados en pacientes con asma o con enfermedad pulmonar obstructiva crónica (EPOC) en comparación con los grupos control del mismo rango etario. El incremento de ambos parámetros se hace más significativo en los pacientes severos de ambos grupos (P < 0,0001) y particularmente en los pacientes con EPOC severo en comparación con los asmáticos severos (P < 0,01). El incremento en los niveles séricos de MMP-9 en relación con severidad es mayor que lo observado para TIMP-1 en ambas patologías. Se concluye que los niveles séricos de MMP-9 y TIMP-1 pueden ser un marcador importante para determinar severidad en estas enfermedades
Serum levels of metalloproteinase (MMP) 9, an enzyme involved in inflammation, and its counterpart, the tissue inhibitor of metalloproteinases type 1 (TIMP-1) are significantly (P < 0.01) increased in patients with asthma or with chronic obstructive pulmonary disease (COPD) as compared with controls of the same age. The increase in both parameters is more significant (P < 0.0001) in severe patients of both groups, and particularly in patients with severe COPD as compared to severe asthmatics (P < 0.01). The increase in serum MMP-9 levels in relation with severity is higher than the observed values for TIMP-1 in both diseases. It is concluded that serum levels of MMP-9 and TIMP-1 may be important markers to establish severity in these diseases
Subject(s)
Humans , Male , Female , Asthma/diagnosis , Pulmonary Disease, Chronic Obstructive/diagnosis , Tissue Inhibitor of Metalloproteinase-1/analysis , Biomarkers/analysis , Biomarkers/blood , Matrix Metalloproteinase 9/analysis , Allergy and ImmunologyABSTRACT
Objective:To investigate the effect of IRH on pulmonary fibrosis and its intervention mechanism.Methods:45 sprague-dawley rats were randomly divided into three groups.The model group and the intervention group were received endotracheal injection of bleomycln to induce pulmonary fibrosis,while the control group were received endotracheal injection of saline.From the second day the intervention group IRH were injected to the stomach,while the other two groups were injected saline instead.On the 7th day,14th day and 28th day the lungs were isolated.The expression of MMP-2 and TIMP-1 was detected by immunohistochemical method respectively.Results:There was only a very weak expression of MMP-2 and TIMP-1 in the control group,there was significantly higher level in model group compared with control group on the 7th day,14th day and 28th day.The IRH group was significantly lower than the model group with the same time points,though it was also obviously higher than the control group all over the period(P
ABSTRACT
Subject(s)
Humans , Carcinoma, Squamous Cell , Cell Differentiation , Immunohistochemistry , Korea , Lymph Nodes , Mouth Neoplasms , Neoplasm Metastasis , Peptide Hydrolases , Prognosis , Radiotherapy , Saints , Tissue Inhibitor of Metalloproteinase-1 , TongueABSTRACT
Objective:To investigate the effects of sodium phenylbutyrate(NaPB) on the matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metalloproteinase-1(TIMP-1) expression and invasive ability of human thyroid follicular carcinoma cell line CGTHW-1.Methods: CGTHW-1 cells were treated with different concentrations of NaPB,then the invasive ability of CGTHW-1 cells was assessed using Transwell assay.The expression of MMP-9 and TIMP-1 was examined by immunocytochemistry staining and RT-PCR in CGTHW-1 cells.Results: After treatment with NaPB(4 mmol/L) for 72 h,CGTHW-1 cells passing the Transwell were significantly reduced[(29.8?1.77) vs(11.00?2.59),P